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It's technically not the oldest botanical garden in the world.
The Botanical Garden of Padua, which was founded in 1545, is arguably the earliest botanical garden in the world. This title is arguable because it's only a year younger than another garden in Italy. While the Orto Botanico di Pisa (Botanical Garden of Pisa) technically holds the distinction of being the oldest botanical garden still in existence, its history of relocation leaves room for Orto botanico di Padova to step in as oldest botanical garden on its original site-a distinction that some feel is important.